Pierre-Henri Puech

Pierre-Henri Puech

Inserm researcher

Publications

updated August 12th, 2024

Pre-prints / submitted

CTRL-C CTRL-V, …, Inkscape import…, we are working on 2 or 3 things… stay tuned !

  • Decoupling individual host response and immune cell engager cytotoxic potency. Cristina Gonzalez Gutierrez, Adrien Aimard, Martine Biarnes-Pélicot, Brigitte Kerfelec, Pierre-Henri Puech, Philippe Robert, Francesco Piazza, Patrick Chames, Laurent Limozin. bioRxiv. doi: link

  • Cellular forces during early spreading of T lymphocytes on ultra-soft substrates. Farah Mustapha, Martine Biarnes-Pelicot, Remy Torro, Kheya Sengupta, Pierre-Henri Puech. bioRxiv. doi: link

Accepted

Working on it…

Books

  • Physique de la matière molle (Dunod, 224p – 2018) de Françoise Brochard-Wyart, Pierre Nassoy, Pierre-Henri Puech. link
  • Essentials of Soft Matter Science (CRC Press, 268p – 2019) de Françoise Brochard-Wyart, Pierre Nassoy, Pierre-Henri Puech. link
  • Chinese traduction of PMM, Dunod is on print (May 2024, see the news section !)

Peer-reviewed articles & reviews

  1. Thierry Galliano, Guillaume Gay, Laurent Limozin, Pierre-Henri Puech*. OT_Analysis: a software for rich analysis of force curves when probing living cells with optical tweezers. Journal of Open Source Software, 8(90), 4877, https://doi.org/10.21105/joss.04877
    • Git / documentation: https://github.com/phpuech/OT_Analysis
    • pip: https://pypi.org/project/OT-Analysis/
    • Documentation, extended: http://otanalysis.free.fr/

  2. Fabio Manca, Gautier Eich, Omar N’Dao, Lucie Normand, Kheya Sengupta, Laurent Limozin, Pierre-Henri Puech. Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction. Scientific Reports 13, Article number: 15652 (2023). https://www.nature.com/articles/s41598-023-42599-9

  3. Mustapha F., Sengupta K, Puech PH, . Mechanical Forces in T cell Biology. Book Chapter, In: Hussein Fayyad Kazan, editor. Immunology and Cancer Biology. Hyderabad, India: Vide Leaf. 2022. https://videleaf.com/mechanical-forces-in-t-cell-biology/

  4. Protocol for measuring weak cellular traction forces using well-controlled ultra-soft polyacrylamide gels. Mustapha F, Sengupta K, Puech PH. STAR Protoc. 2022 Jan 28;3(1):101133. doi: 10.1016/j.xpro.2022.101133

  5. Mechanotransduction as a major driver of cell behaviour: mechanisms, and relevance to cell organization and future research Puech Pierre-Henri* and Bongrand Pierre*. Open Biol. 2021 Nov;11(11):210256. doi: 10.1098/rsob.210256

  6. iASPP contributes to cortex tension, astral microtubule capture and mitotic spindle positioning via EB1 and Myo1. Aurélie Mangon, Danièle Salaün, Mohamed Lala Bouali, Sabine Quitard, Daniel Isnardon, Stéphane Audebert, Pierre-Henri-Puech, Pascal Verdier-Pinard et Ali Badache, J Cell Biol. 2021 Dec 6;220(12):e202012002. doi: 10.1083/jcb.202012002

  7. Controlling T cells shape, mechanics and activation by micropatterning. A. Sadoun, M. Biarnes-Pelicot, L. Ghesquiere-Dierickx, A. Wu, O. Théodoly, L. Limozin, Y. Hamon, P.-H. Puech, Sci Rep. 2021 Mar 24;11(1):6783. doi: 10.1038/s41598-021-86133-1

  8. Single-cell immuno-mechanics: rapid viscoelastic changes are a hall-mark of early leukocyte activation Alexandra Zak, Sara Violeta Merino Cortés, Anaïs Sadoun, Farah Mustapha, Avin Babataheri, Stéphanie Dogniaux, Sophie Dupré-Crochet, Elodie Hudik, Hai-Tao He, Abdul I Barakat, Yolanda R Carrasco, Yannick Hamon, Pierre-Henri Puech, Claire Hivroz, Oliver Nüsse, Julien Husson, Biophys J. 2021 May 4;120(9):1692-1704. doi: 10.1016/j.bpj.2021.02.042

  9. Determination by Relaxation Tests of the Mechanical Properties of Soft Polyacrylamide Gels Made for Mechanobiology Studies. Daniel Pérez-Calixto , Samuel Amat-Shapiro , Diego Zamarrón-Hernández , Genaro Vázquez-Victorio , Pierre-Henri Puech, Mathieu Hautefeuille, Polymers (Basel). 2021 Feb 20;13(4):629. doi: 10.3390/polym13040629

  10. Mechanical adaptation of monocytes in model lung capillary networks. Dupire J, Puech PH, Helfer E, Viallat A. Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14798-14804.

  11. Dynamics of Toxoplasma gondii Oocyst Phagocytosis by Macrophages. Omar Ndao, Pierre-Henri Puech, Camille Bérard, Laurent Limozin, Sameh Rabhi, Nadine Azas, Jitender P. Dubey, Aurélien Dumètre. Front. Cell. Infect. Microbiol., 19 May 2020 https://doi.org/10.3389/fcimb.2020.00207

  12. Membrane Organization and Physical Regulation of Lymphocyte Antigen Receptors: A Biophysicist’s Perspective. Limozin L, Puech PH .J Membr Biol. 2019 Jul 27. doi: 10.1007/s00232-019-00085-2

  13. Wahl A, Dinet C, Dillard P, Nassereddine A, Puech PH, Limozin L, Sengupta K. Biphasic mechanosensitivity of T cell receptor-mediated spreading of lymphocytes. Proc Natl Acad Sci U S A. 2019 Mar 8. pii: 201811516. doi: 10.1073/pnas.1811516116

  14. Wesley Freppel, David J.P. Ferguson, Karen Shapiro, Jitender P. Dubey, Pierre-Henri Puech, Aurélien Dumètre. Structure, composition, and roles of the Toxoplasma gondii oocyst and sporocyst walls. The Cell Surface, 2018 (5), 100016

  15. Dejardin MJ, Hammerle A, Sadoun A, Hamon Y, Puech PH, Sengupta K, Limozin L Lamellipod reconstruction by three dimensional reflection interference contrast nanoscopy (3D-RICN). Nanoletters (2018) Oct 10;18(10):6544-6550

  16. Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Microscopy Based Force Measurements. Sadoun A, Puech PH*. Methods Mol Biol. 2017;1557:139-151. doi: 10.1007/978-1-4939-6780-3_14.

  17. Macrophages facilitate the excystation and differentiation of Toxoplasma gondii sporozoites into tachyzoites following oocyst internalisation. W. Freppel, PH. Puech, D. J. P. Ferguson, N. Azas, J. P. Dubey, A. Dumètre. Sci Rep. 2016 Sep 19;6:33654. doi: 10.1038/srep33654.

  18. Dynamic monitoring of cell mechanical properties using profile microindentation. Guillou L, Babataheri A, Puech PH, Barakat AI, Husson J. Sci Rep. 2016 Feb 9;6:21529. doi: 10.1038/srep21529.

  19. Synchronizing atomic force microscopy force mode and fluorescence microscopy in real time for immune cell stimulation and activation studies. Cazaux S, Sadoun A, Biarnes-Pelicot M, Martinez M, Obeid S, Bongrand P, Limozin L, Puech PH*. Ultramicroscopy. 2016 Jan;160:168-81. doi: 10.1016/j.ultramic.2015.10.014. Epub 2015 Oct 19.

  20. Aurélien Dumètre, Jitender P. Dubey, David J. P. Ferguson, Pierre Bongrand, Nadine Azas, and Pierre-Henri Puech*, Mechanics of the Toxoplasma gondii oocyst wall , PNAS 2013 110 (28) 11535-11540

  21. Lo Schiavo V, P Robert, Z Mishal, PH Puech F Gentile, P Decuzzi, P Bongrand, L Limozin. Transient adhesion mediated by ligand-receptor interaction on surfaces of variable nanotopography. Int. J. Nanotechnology 2013 Vol.10 No.5/6/7, DOI: 10.1504/IJNT.2013.053512

  22. Dumetre, Aurelien; Aubert, Dominique; Puech, Pierre-Henri; et al. Interaction Forces Drive the Environmental Transmission of Pathogenic Protozoa. APPLIED AND ENVIRONMENTAL MICROBIOLOGY 2012, 78(4) 905:912

  23. Pierre-Henri Puech*, Damien Nevoltris, Philippe Robert, Laurent Limozin, Claude Boyer and Pierre Bongrand, Force measurements of TCR / pMHC recognition at T cell surface. PloS ONE 2011;6(7):e22344

  24. Hampoelz B, Azou-Gros Y, Fabre R, Markova O, Puech PH, Lecuit T. Microtubule-induced nuclear envelope fluctuations control chromatin dynamics in Drosophila embryos. DEVELOPMENT. 2011 Aug;138(16):3377-86 – Correction (wrong author affiliation) in Development. 2017 Feb 15;144(4):732. doi: 10.1242/dev.149575.

  25. Nina Milosavljevic, Christophe Duranton, Nadir Djerbi, Pierre-Henri Puech, Pierre Gounon, Dominique Lagadic-Gossmann, Marie Thérèese Dimanche Boitrel, Michel Tauc, Laurent Counillon et Mallorie Poet, Cisplatin inhibits mechanosensitive ion transporters and channels. Implications for cell homeostasis and pain sensation. Cancer Res. 2010 Oct 1;70(19):7514-22. doi: 10.1158/0008-5472

  26. Atomic Force Microscopy : A Versatile Tool for Studying Cell Morphology, Adhesion and Mechanics Franz CM, Puech PH* CELLULAR AND MOLECULAR BIOENGINEERING 1(4) :1865-5025 2008

  27. Tuning the formation and rupture of single ligand-receptor bonds by hyaluronan-induced repulsion Robert P, Sengupta K, Puech PH, Bongrand P, Limozin L BIOPHYSICAL JOURNAL Volume : 95 Issue : 8 Pages : 3999-4012 Published : OCT 15 2008

  28. BCR/ABL expression of myeloid progenitors increases beta 1-integrin mediated adhesion to stromal cells Fierro FA, Taubenberger A, Puech PH, et al. JOURNAL OF MOLECULAR BIOLOGY Volume : 377 Issue : 4 Pages : 1082-1093 Published : APR 4 2008

  29. Tensile forces govern germ-layer organization in zebrafish Krieg M, Arboleda-Estudillo Y, Puech PH, et al. NATURE CELL BIOLOGY Volume : 10 Issue : 4 Pages : 429-U122 Published : APR 2008

  30. Studying integrin-mediated cell adhesion at the single-molecule level using AFM force spectroscopy. Franz CM, Taubenberger A, Puech PH, Muller DJ. SCIENCE STKE. 2007 Oct 2 ;2007(406) :pl5.

  31. Revealing early steps of α2β1 integrin-mediated adhesion to collagen type I by using single-cell force spectroscopy Taubenberger A, Cisneros DA, Friedrichs J, et al. MOLECULAR BIOLOGY OF THE CELL Volume : 18 Issue : 5 Pages : 1634-1644 Published : MAY 2007

  32. A new perspective on cell-cell adhesion Poole K, Puech PH, Muller D CYTOMETRY PART A Volume : 71A Issue : 1 Pages : 53-53 Published : JAN 2007

  33. A new technical approach to quantify cell-cell adhesion forces by AFM Puech PH, Poole K, Knebel D, et al. ULTRAMICROSCOPY Volume : 106 Issue : 8-9 Pages : 637-644 Published : JUN-JUL 2006

  34. Measuring cell adhesion forces of primary gastrulating cells from zebrafish using atomic force mi- croscopy Puech PH, Taubenberger A, Ulrich F, et al. JOURNAL OF CELL SCIENCE Volume : 118 Issue : 18 Pages : 4199-4206 Published : SEP 15 2005

  35. Wnt11 functions in gastrulation by controlling cell cohesion through Rab5c and E-cadherin Ulrich F, Krieg M, Schotz EM, et al. DEVELOPMENTAL CELL Volume : 9 Issue : 4 Pages : 555-564 Published : OCT 2005

  36. Dynamics of vesicle adhesion : spreading vs. dewetting coupled to binder diffusion P.-H. Puech ; V. As- kovic ; P. -G. De Gennes ; F. Brochard-Wyart BIOPHYSICAL REVIEWS AND LETTERS, Volume 1, Issue 14 Pages : 85 – 95; 2006

  37. Membrane tensiometer for heavy giant vesicles Puech PH, Brochard-Wyart F EUROPEAN PHYSICAL JOURNAL E Volume : 15 Issue : 2 Pages : 127-132 Published : OCT 2004

  38. Adhesion between giant vesicles and supported bilayers decorated with chelated E-cadherin fragments Puech PH, Feracci H, Brochard-Wya F LANGMUIR Volume : 20 Issue : 22 Pages : 9763-9768 Published : OCT 26 2004

  39. Adhesion induced by mobile stickers : A list of scenarios de Gennes PG, Puech PH, Brochard-Wyart F LANGMUIR Volume : 19 Issue : 17 Pages : 7112-7119 Published : AUG 19 2003

  40. Interaction of polyelectrolyte coated beads with phospholipid vesicles Fery A, Moya S, Puech PH, et al. COMPTES RENDUS PHYSIQUE Volume : 4 Issue : 2 Pages : 259-264 Published : MAR 2003

  41. Cascades of transient pores in giant vesicles : Line tension and transport Karatekin E, Sandre O, Guitouni H, et al. BIOPHYSICAL JOURNAL Volume : 84 Issue : 3 Pages : 1734-1749 Published : MAR 2003

  42. Line thermodynamics : Adsorption at a membrane edge Puech PH, Borghi N, Karatekin E, et al. PHYSICAL REVIEW LETTERS Volume : 90 Issue : 12 Article Number : 128304 Published : MAR 28 2003

  43. Giant vesicles under flows : Extrusion and retraction of tubes Rossier O, Cuvelier D, Borghi N, et al. LANGMUIR Volume : 19 Issue : 3 Pages : 575-584 Published : FEB 4 2003

Conference proceedings

  1. Tethered Giant Vesicles under Flows : Extrusion of Tubes Borghi, N; Rossier,O; Puech PH; Buguin, A ; Brochard-Wyart, F Proceedings of the First International Congress on Bio-Nanointerfaces (2003) – Tokyo (Japan)

Technical notes

  1. Dissecting T-cell mechanosensing at molecular and cellular scales : The use of nano-force tools by A. Sadoun et al. 2020, Wiley Analytical Science. link

  2. Dissecting T cell Mechanosensing

  3. JPK User Story

  4. JPK CellHesion, 2

  5. JPK CellHesion, 1